Recombinant Human bFGF

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產品型號：abs00877
廠商性質：生產廠家
更新時間：2025-06-11
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細胞生物學

重組蛋白原代細胞分離細胞培養細胞檢測細胞/組織處理外泌體研究其它動物血清 3D/類器官培養

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詳細介紹

品牌	absin	貨號	abs00877
規格	10ug	供貨周期	一周

概述
種屬	Human
形態	Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.
來源	Escherichia coli.
氨基酸序列	MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
內毒素水平	Less than 1 EU/µg of Recombinant HumanbFGF as determined by LAL method.
活性	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg.
Accession #	P09038
預期分子量	Approximay 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.

性能
溶解方法	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
純度	> 96 % by SDS-PAGE and HPLC analyses.
Gene IDs	2247

參考文獻
1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.<br/>2. Gospodarowicz D. 1974. Nature. 249:123-7.<br/>3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.<br/>4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.<br/>5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.<br/>6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.<br/>7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.

參考文獻

1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6. 2. Gospodarowicz D. 1974. Nature. 249:123-7. 3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49. 4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57. 6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29. 7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.

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Recombinant Human bFGF

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